Essay --

A prokaryote is a basic unicellular cell typically chracterized by having no defined nucleus. Examples of prokaryotes are typically given as orgamins that belong to the Bacteria and Archaea domains (Campbell 13). Furthermore, prokaryotes are very similar to eukaryotes except for the fact that they don't have a nucleous but a nucleoid and that prokaryotes are smaller than eukaryotes (Campbell 98). By the definition of a prokaryote and process of elimination it is easy to find the correct answer which is D. A)Is incorrect because all cells require ribosomes (Module 14, slide 4) . Ribosomes are important since they make proteins which are part of the basic building blocks of life (Campbell 102). B) The plasma membrane on the outside of the cell is required since it separates the innards from the ouside (Module 14, slide 4). They are can perform this fuction since they are made of a lipid bilayer with hydophilic and hydrophobic parts to keep the inside of the cell prrotected from the outside environment (Campbell 99). C) The photo of prokaryotes on slide 6 of module 14 shows that it ...

The Controversy Over Home Schooling Essay -- Essays Papers

The Controversy Over Home Schooling In today’s society, the controversy over home schooling is a pertinent issue. Home schooling is known as the instruction of a pupil in an educational program outside of an established school, usually under the tutoring of the parent or guardian. Several debates exist over this current topic. Some have reasonable arguments in favor of home schooling, while many disagree with its process and the motives behind it. Both arguments have valid points that must be explored. Millions of people across the country believe that teaching children at home deprives them of the average childhood experience. They argue that these children are ostracized because they are left out of many activities that can only be experienced in a traditional school. For example, field trips are common among elementary school students. Home schooled children are not given the opportunity to attend these important educational trips because they are kept at home and taught by their parents. Another instance is extracurricular activities. Many s...

Rational Unified Process :: essays research papers

Introduction This paper has the intention to explain what Rational Unified Process (RUP) is like an IBM product and a CASE tool. After is explained what phases it has, what are the most common extensions thus what are its workflows more used. The Rational Unified Process (RUP) is a software design method created by the Rational Software Corporation and now is part of IBM developer software. This paper describes how to deploy software effectively. The Rational Unified Process (RUP) use commercially proven techniques, and is a heavy weight process, and hence particularly applicable to larger software development teams working on large projects. Rational Unified Process (RUP) Rational Unified Process (RUP) is an object-oriented and Web-enabled program development methodology. RUP would be taken like an online mentor that provides guidelines, templates, and examples for all aspects and stages of program development. RUP is a comprehensive software engineering tool that combine the procedural aspects of development (such as defined stages, techniques, and practices) with other components of development (such as documents, models, manuals, code, and so on) within a unifying framework. The RUP defines the following guidelines and templates for team members to follow during a product’s lifecycle: Develop Software Iteratively Given the time, it takes to develop large sophisticated software systems it not possible to define the problem and build the solution in a single step. Requirements will often change throughout a projects development, due to architectural constraints, customer’s needs or a greater understanding of the original problem. Iteration allows greater understanding of a project through successive refinements and addresses a projects highest risk items at every stage of its lifecycle. Ideally each iteration ends up with an executable release – this helps reduce a projects risk profile, allows greater customer feedback and help developers stay focused. Manage Requirements A documentation framework is essential for any large project; hence, RUP describes how to document functionality, constraints, design decisions and business requirements. Use Cases and Scenarios, are examples of artifacts prescribed by the process and have been found to be very effective at both capturing functional requirements and providing coherent threads throughout the development and deployment of the system. Use component based architecture Component Based Architecture creates a system that is easily extensible, promotes software reuse and intuitively understandable. A component often relates to an object in Object Orientated Programming. The RUP provides a systematic way to build this kind of system, focusing on producing an early executable architecture before committing full resources on a project.

Imagery of Snakes in Macbeth Essay examples -- European Literature

In the biblical book of Genesis, Eve is tempted by a serpent to commit an ungodly act of eating a forbidden fruit. As a result of the snake's temptation, Eve eats the fruit, resulting in the presence of worldwide sin and the downfall of perfection. From this story of Adam and Eve, the figure of the snake has become generally associated with temptation, evil, and cynicism. William Shakespeare employs this symbolic imagery of snakes in Macbeth, to enhance the story of the rise and fall of Macbeth's power. For poetic and playwright purposes, Shakespeare uses serpentine imagery to reveal the malevolence of characters, and portray the threatening position of the throne, all while provoking a heightened emotion of fear and tragedy from the audience. The first image of the snake is revealed during the initial arrival of evil. In act one, scene five, Lady Macbeth cries out for evil to fulfill her, so that she can posses the power to commit acts of treason. She describes this process as "unsexing" herself. "Come to my woman's breasts and take my milk for gall, you murd'ring ministers" (1.5.48-49). In ancient history it was believed that the venom of serpents was produced from their gall1. The process of "unsexing" herself could, as a result, be perceived as a metaphorical transformation into a snakelike creature. The "unsexing" process is an important part of the evil and complexity that Shakespeare was attempting to develop throughout the story, in order to invoke emotion from the audience. This "unsexing" process not only brings about a theme of witchcraft, but it also introduces a complex situation that the character must face: whether or not to give up good and loyalty for personal ambition. This decision is one that p... ...ke." The imagery of snakes throughout the story of Macbeth contributes to the mood and poetic prose of Shakespeare. Yet more importantly, the snake is a symbolic figure for malevolence and treachery. Shakespeare uses the "snake" to enhance the quality of his characters, and provide insight into their malevolence. The serpentine image also provokes emotion. As the story of Macbeth is a tragedy, the snake images provide a dark contrasting mood to the play, designed to heighten the sense of fear from the audience. People are generally afraid snakes, and Shakespeare uses this object as a tool to relate certain fears of the characters to the audience. Shakespeare is able to develop his characters while capturing the emotion of his audience all by his use of serpentine imagery. Works cited 1. Oxford English Dictionary. Search: gall.

Nicotine Addiction :: Research Analysis

Nicotine addiction Cigarette addiction is responsible for over four million deaths every year. The question most people ask is why don’t people just put down their cigarettes? Well, the answer to that often asked question is nicotine. Nicotine is a neurotransmitter that targets certain receptors in the brain. It is a chemical messenger that induces feelings of pleasure. When someone takes a hit off of a cigarette, they ingest the harmful chemicals that can cause cancer and other serious health threats. They only inhale these chemicals to get one thing and one thing only, nicotine. Curing addiction becomes harder and harder with each puff of a cigarette one takes. When nicotine is consumed, it communicates with the brain and is absorbed into receptor molecules, Doctors for years have undergone hundreds of experimental studies on the human brain to help understand just how these chemicals neurologically affect the brain, and which sectors of the brain are affected. This goal has not yet been accomplished due to the large amounts of receptor cells in the brain. In a study performed at the California Institute of Technology performed by Andrew Tapper, Professor Allan Collins from the University of Colorado, Several colleagues, and Henry A. Lester, the Caltech University Bren Professor of Biology revealed that nicotine affects a small subunit of the brain called alpha four. This subunit of the brain increases levels of pleasure, response, sensitization, and increases tolerance of multiple doses of the drug nicotine. Many people do not know what nicotine does inside the brain. Here’s how it works when the nicotine reacts with the cells in the brain. It creates nerve impulse that jump chemically across a gap between two different nerve cells. This action is called a synapse. The neurotransmitter acetylcholine is used in this process and is used to affect specified receptors in the brain targeting post-synaptic nerve cells. After this process, the feel good brain messenger, dopamine, is released. This chemical is released into the brain creating an extreme high. The acetylcholine is then supposed to diminish the dopamine after completing its task. The drug disguises itself as acetylcholine causing the process of dopamine release to last for minutes rather than milliseconds. In several lab studies performed on mice in the 1990’s, results concluded that nicotine also affects the subunits of the brain labeled beta two. Knowing how this drug affects many different subunits shows just how complicated it is to find a cure for addiction to this drug.

Politics in Joseph Conrads Heart of Darkness :: Heart Darkness essays

Politics in Heart Of Darkness Anyone can read Heart Of Darkness and easily sense the attitude of Conrad toward English politics. Many times throughout Heart Of Darkness Conrad points out the pointlessness and savagery of English colonization. Conrad also comments a bit on society as a whole. With these two ideas added to the book, there is no wonder of why Heart of Darkness is such a touching novel. Through several examples, Conrad often shows the pointlessness and savagery of the English colonization in Africa. Probably the first instance of this is when Marlow comes up to the French-man who is "shelling the bush". In this scene, the French see something move and so they start shelling it for that reason. The shelling really does no good; if fact, it probably does not even kill what is out there. This represents what the English are doing in a way -- they are trying to conquer a land by shelling it to death and by trying to kill all the people who live there. The next example that Conrad gives is when he sees the black guard, who is leading the black slaves in a chain gang, straighten up when he sees a white man. What this shows is how everyone tries to look better than they are when they are in front of a supposed superior person. Also it shows that if a person can suck up enough -- and sometimes betray their own people -- they can move up in the world. Probably the biggest example of the pointless of colonization is when Marlow is walking around and he sees big holes just around, a train and tools rusting to pieces, and when he hears blasts that seem to do nothing. What this shows is that the English presence in Africa does no good but create a empty hole in it (the big hole), that the English are just was wasting their time and money on a needless project (the rusting objects), and that the English seem to do nothing in Africa (the pointless blasting). One the final examples that he gives is the manager. This manager is all dressed up in "proper" clothes, must have everything in a perfect order, and complains about the sick man in the corner of the room. This symbolizes how uncaring the English are in their pursuit for ivory.

Cell and Molecular Biology

Cell and Molecular Biology Final Name: Hadeel Binomar30 pts. Protein misfolding can be an aspect of several different human disorders, including cystic fibrosis, Alzheimer's disease, and atherosclerosis. Many times, the misfolded protein is a membrane protein. In fact, a type of diabetes insipidus results from a mutation in the G-protein-coupled vasopressin 2 receptor that prevents the protein from making it to the cell membrane A) Describe how this type of receptor would normally get targeted to the membrane (from the beginning of translation) and B) then propose one mechanism by which the mutation could cause a lack of proper targeting. One of the most important protein's target is G protein-coupled receptors, several signaling mechanisms depend on this type of receptor to change both internal and external stimuli to the intracellular responses. Basically, one of the G couple receptor subfamilies is G-coupled Vasopressin-2- Receptor (V2R), and this receptor is going through a strict quality control process at the endoplasmic reticulum, which presents the only correctly folded protein to gets through the secretory pathway. The primary function of the V2 receptor is to activate the attached G protein that bound to the ? subunit then phosphorylated to GTP. The G protein couple receptor then activates the enzyme adenylate cyclase that catalyzes the reaction in the ER and forms cAMP from ATP. After that, cAMP acts as a second messenger and activates a protein kinase that phosphorylates the integral membrane proteins on the cell surface. Moreover, the secretory pathway organelles' and the plasma membrane both are first introduced into the Endoplasmic reticulum, and the co-translationally proteins that can cross the ER are synthesized by the ribosome first then binds by chaperones to gets moves to the ER surface using GTP that allows them to move toward the receptor then release it. The soluble proteins and the integral membrane proteins as I mentioned above, can be targeted through the ER and then translocated by the same mechanism.Further, several mutations occurring in the transmembrane region which affect the structure of the protein. These are multiple mutation sites, such as mutations occurring in the amino acid residues which was acting as a causative agent for human disease. Also, there are other mutations occurring on the single site position that will affect the translation mechanism and cause many human diseases like: cystic fibrosis, Alzheimer's disease, and atherosclerosis that proves the function of V2R protein which plays important role in the translation during the protein folding process. One of the mechanism that may a reason for lacking the proper target protein is when the mutations of the CFTR gene occurring and affect the function of the chloride ion channels and cause defect in the protein sequences which lead to the production of diseases and misfolded of the proteins that are unable to recognize their functional destinations. Otherwise, Lack of the stop signals is another issue that prevents the protein from getting into the surface of the ER, also called the non-stop decay cellular pathway, because lack of this stop signals prevents mRNA from synthesis and translate the proteins, these consider as a point mutation that inhibits the essential stop codons. 30 pts. Describe the experiment shown in figure 3 from the paper we discussed in class (Miller et al, 2003). (A) In your description, consider the following questions: Why did they do it? How did they do it? What did they learn? (B) Diagram the results that you would expect to see in Lanes T, 1, 2, 3, and 4, if the amino acid signal DID in the protein Gap1p was mutated to random amino acids and tell why; and (C) Give two possible (different) results that might occur if the amino acid signal LxxLE in Bet1p was mutated to the amino acids DID (which are the signal in Gap1p). Diagram the results expected in Lanes T, 1, 2, 3, and 4 and explain why you predicted this result for each case.left20840701.A001.A4467225201739500The experiment was performed to study the role of cargo binding domain of Sec24p in the process of protein sorting. To perform this study, both mutant and wild types subunit Sec23/24p and Sec23/24L616W were harvested from microsomal membranes. The immunoblotting assay performed to quantify cargo molecules using radio-labeled secondary antibodies. Comparison of the mutant subunit with wild type illustrated omission of some molecules in mutant one, these molecules were cargo protein molecules. While some of them are packed in a way that similar to the wild-type. It meant that there were some signals that remained unaffected although mutation was there. Unexpectedly, it was found that in these unaffected molecules of mutation in Sec23/24p, packaging was better than the wild type. Further, they found the proteins that were highly affected had Bet1p and Gap1p/Sys1p chimera, because they completely depending on the Sys1p di-acidic of COPII vesicles. If the amino acid signal DID in the protein Gap1p was mutated to random amino acids, the resulting bands would be seen as in figure 1. A because the amino acids might be present in all the lanes. And Gap1p is required for di-acidic motifs to fuse together with the COPII vesicles at the mutated domain. Also, Sys1 peptide is not involved in Sec 23/24p hence its mutation is not contained in a di-acidic motif. If the signal LxxLE was mutated to the DID, the expected Bet1p packaging would be shown in figure 2. A as not be seen in lanes containing Bet1p because the amino acid signal LxxLE does not exit after mutating to amino acids DID. The second probability for the Bet1p would be expected to bind with the vesicle for packaging when it interacts with di-acidic motifs which is needed as shown in figure 2.B290512515621000581025113030004781550412752.B002.B6286501346202.A002.A20 pts. Explain the experiment shown in Figure 5C from the Shen et al. (2018) paper on the phosphorylation of CDC25 that we discussed in class. In addition, be sure to also address the following: A) why did they do this experiment (the central issue); B) what did they learn; and C) what is another control experiment they could have done? D) Would these results change if you added a constitutively active form of LKB1 to the reaction? If so, how and why? A) Overall, the main purpose of this paper is to explain how the division of cells is highly regulated such that cells that fail to pass some specific stage-based tests cannot advance to the proceeding stages. In this particular experiment, HeLa cells were subjected to chemicals that are known to activate the enzyme AMPK. Also, the main catabolic processes that are involved to generate energy for cells to transition from G1/G2 were determined by the application of radiochemical approaches, the experiment required to approve how highly conserved cellular energy sensor can significantly delay mitosis entry and activation of AMP-activated protein kinase (AMPK). Further, Wee 1 family inactivates the cell cycle G2/M Phase which is controlled by Cdc3/cyclic B (mitotic cyclin-dependent kinase complex). They found that AMPK-dependents phosphorylation of CDC25C arranges a metabolic control point for the M-phase transition and the cell cycle phase G2. B) Also, they learned that suppression of Wee 1 or acute induction partially reinstates mitosis ingress in the circumstance of activated protein kinase (AMPK). This experiment showed that when Phosphorylates Cdc25 is in a distributive and disordered state, it results into ultra-sensitivity in protein phosphorylation. C) For another control experiment, they might try exposing the U2OS (cell line) clones conditionally exhibiting Cdc25A etoposide. That will help to test whether Cdc25A degradation is significant for the G2 control point or not.D) If they add an active form of LKB1 to the reaction, the results will not change, because in cells LKBI activity inhibits AMPK activation in response to different stimulations. Also, LKBI is lost upon consistent isolation and therefore no effects of phosphates observed.20 pts. Briefly describe the role of cyclin-CDK in the cell cycle and give an example of positive and negative control in this system. Also, describe how cell cycle regulation links to the stimulation of apoptosis at the molecular level. In the beginning, the cell cycle depends on many basic factors that control the regulation process starting from the signal transcription molecules, growth factors and the Cyclin Dependent Kinases enzymes include the checkpoints which control the transition process between the cell cycle phases by binding to the cyclin proteins CDKs then phosphorylate other proteins to transfer from one phase to another. The role of transcription factors is to turn on the signals for gene expression, DNA replication, and cell divisions. As an example of CDKs, cyclin-dependent kinase 1 CDK1 is a cell division cycle protein homolog 2, that has a primary role in human cancer cells because CDK1 rather than any types of CDKs is fatal to the mutated version of MYC- dependent cancer that leads to a depletion of oncogenes like (Fos and Jun) in human cancer cells. Fos and Jun are combined forms of the transcription factor called AP1 that activates the delayed response genes such as cyclin D and CDK4. Recent studies reveal that the reason for the MYC breast cancer cells duplation is targeting CDF1 exhibit any other CDKs cell lines. Also, CDK1 inhibition can control and target the cancer cells in human and both phosphorylation and expression of MYC during the cell cycle process. To initiate intracellular signaling pathways and stimulate the cell cycle entry, mitogens substance bind to the cell surface receptor with the activation form of GTPase Ras that activates MAP kinase cascade. That will lead to the expression of encoding gene of the transcription regulatory protein like MYC. Moreover, E2F transcription factor is a target for cyclin D and CDKs that stimulate proteins expression to initiate S phase, also E2F regulated by the tumor suppressor gene Rb (Retinoblastoma protein). At early G1 Phase, Rb protein combined with E2f to form the histone deacetylases protein that remains chromatic condensed, then cyclin kinase phosphorylates Rb protein, causing it to disassociate from E2F. That allows recruitment of histone acetylates, which decondense the chromatin and helps transcription complexes to form in G1 and S phase and to form a positive feedback. For the negative control, if there are no growth factors present to stimulate the synthesis of Cyclin D in the new cell, Rb phosphorylated will turn off, and Rb will rebind to E2F, then the cell cycle will stop and that will lead to many negative results like prevent it to bind with DNA, or DNA damage and cells will return to the G0 phase. Apoptosis is a consequence of DNA damage; if the damage is not repaired, the cell cycle will divert towards apoptosis, otherwise, if the cell has DNA defects and fail to undergo apoptosis, that will change to the cancer cell. G1 phase regulators such as P53 and E2F are essential to promote the cell regulations and eliminate any damage or abnormal changes during the cell cycle. P53 has a primary function to prevent any mutation in DNA during cell progression, so any defect in P53 will lead to cancer, and it's usually mutated in cancer cells. Further, Rb protein considers as a tumor suppressor and also promotes apoptosis. Additionally, most human cancers have inactive Rb protein, either mutated Rb or the non-phosphorylated (inactivated form) of Rb protein